Localization of an O‐glycosylation site in the α‐subunit of the human platelet integrin GPIIb/IIIa involved in Baka (HPA‐3a) alloantigen expression
- 9 August 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 328 (1-2) , 30-34
- https://doi.org/10.1016/0014-5793(93)80959-x
Abstract
The human alloantigen system Bak a/b is associated with a Ile 843→ Ser replacement on platelet glycoprotein IIb, the α-subunit of the integrin receptor for fibrinogen (GPIIb/IIIa). Recent immunological studies indicate that sialylated oligosaccharide chain(s) are also implicated in expression of the Baka determinant. Here we show that the GPIIb fragment 704–856 contains the whole Bak a epitope, and that chemical cleavage of a single O-linked oligosaccharide chain within this GPIIb domain correlates with the loss of its anti-Baka antibodies binding ability. Ser847 was identified as the O-glycosylation site. Therefore, our results show that the Ser847 modification is responsible for the expression of the GPIIb-specific Baka alloantigen, and provide thus a link between the molecular biology and the immunologie observationsKeywords
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