Micronuclear DNA sequences ofOxytricha fallaxhomologous to the macronuclear inverted terminal repeat
Open Access
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (9) , 2911-2924
- https://doi.org/10.1093/nar/10.9.2911
Abstract
The macronucleus of the protozoan Oxytricha fallax is generated from a micronucleus following conjugation. While the micronucleus contains high molecular weight DNA, the macronucleus contains only short linear DNA molecules which all end in the same 20 bp inverted terminal repeat (Ma-ITR). The Ma-ITR was radioactively labeled and purified for use as a probe 1n hybridizations to micronuclear and macronuclear DNA. Sequences homologous to the Ma-ITR were detected in micronuclear DNA. The copy number of the repeat in the micronuclear genome is approximately that required to encode the macronuclear DNA termini. The micronuclear copies are found embedded in repeated long sequence blocks.Keywords
This publication has 22 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- An irregular satellite sequence is found at the termini of the linear extrachromosomal rDNA in Dictyostelium discoideumCell, 1981
- Sequence-specific fragmentation of macronuclear DNA in a holotrichous ciliateCell, 1981
- A family of inverted repeat sequences and specific single-strand gaps at the termini of the Physarum rDNA palindromeCell, 1980
- Inverted terminal repeat sequence in the macronuclear DNA of Stylonychia pustulataGene, 1980
- Isolation and mapping of the rRNA genes in the macronucleus of Oxytricha fallaxChromosoma, 1980
- A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in TetrahymenaJournal of Molecular Biology, 1978
- The Gene-size DNA Molecules in OxytrichaCold Spring Harbor Symposia on Quantitative Biology, 1978
- Amplification and characterization of a β-globin gene synthesized in vitroCell, 1976
- DNA of ciliated protozoa: DNA sequence diminution during macronuclear development of oxytrichaCell, 1976