Biotypes of Haemophilus encountered in clinical laboratories

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Abstract
The biochemical characteristics of 464 strains of H. influenzae and 83 strains of H. parainfluenzae isolated over an 18 mo. period are described. Of 22 characteristics obtained, only 6 were necessary to biochemically identify and biotype the isolates. The key substrates or tests were urease, ornithine, indole, o-nitrophenyl-.beta.-D-galactopyranoside, sucrose and xylose. Five biotypes of H. influenzae and 4 of H. parainfluenzae were commonly recognized. Some strains were encountered which could not be accommodated in the recognized taxa but which constituted separate biotypes of the 2 species. H. influenzae biotype I was recovered principally from blood, CSF, and upper respiratory secretions, and biotypes II and III were recovered from eye and sputum cultures. Biotype I was recovered primarily from children less than 1 yr of age, whereas biotypes II and III were from persons 1-5 yr old and from those over 20 yr of age. Multiple isolates recovered from the same patient were almost always of the same biotype. Strains of H. parainfluenzae were isolated primarily from sputum, with others being isolated from body sources such as dental abscesses, gastric aspirates and peritoneal fluid. An inverse relationship was noticed between hemolysis and mannose fermentation among H. parainfluenzae biotype III strains, whereas the relationship was absent among the other biotypes.

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