DISTRIBUTION OF THE NUCLEASES OF THE MICROSOMAL FRACTION OF RAT LIVER BETWEEN RIBOSOMES AND ENDOPLASMIC MEMBRANES
- 1 February 1966
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 44 (2) , 273-279
- https://doi.org/10.1139/o66-030
Abstract
The distribution of nucleases of the microsomal fraction of rat liver between ribosomes and endoplasmic membranes was studied. The results show that the ribosomes contained a ribonuclease (RNase) inhibited by Mg++ions but activated by ethylenediaminetetraacetic acid (EDTA). Furthermore the degradation products of this enzyme were found to possess a 3′-phosphomonoester end group. The endoplasmic membranes contained a 5′-RNase that could degrade the purine—purine linkage of polyadenylic acid, and a 5′-phosphodiesterase.This publication has 13 references indexed in Scilit:
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