Rapid screening for plasmid DNA
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 151 (2) , 175-179
- https://doi.org/10.1007/bf00338692
Abstract
A procedure is described for demonstrating plasmid DNA and its molecular weight, based on rate zonal centrifugation of unlabelled DNA in neutral sucrose gradients containing a low concentration of ethidium bromide. Each DNA species is then visualized as a discrete fluorescent band when the centrifuge tube is illuminated with ultra-violet light. Plasmids exist as closed circular and as relaxed circular molecules, which sediment separately, but during preparation of lysates, closed circular molecules are nicked so that each plasmid forms only a single band of relaxed circles within the gradient.Keywords
This publication has 26 references indexed in Scilit:
- Problems in the extraction of DNA when utilizing pancreatic RNAase and pronaseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- Two major groups of colicin factors: Their evolutionary significanceMolecular Genetics and Genomics, 1973
- Small polydisperse circular DNA of HeLa cellsJournal of Molecular Biology, 1972
- New calibration correlations for molecular weights of circular DNA: The molecular weight of the DNA of an oncogenic papova virus of the Syrian hamsterBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Partial denaturation of thymine- and 5-bromouracil-containing λ DNA in alkaliJournal of Molecular Biology, 1970
- Simplified quantitative electron microscopy of biopolymersBiopolymers, 1970
- Characterization of multiple circular DNA forms of colicinogenic factor E1 from Proteus mirabilisBiochemistry, 1968
- The interaction of closed circular DNA with intercalative dyesJournal of Molecular Biology, 1968
- A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.Proceedings of the National Academy of Sciences, 1967
- Sedimentation Rate as a Measure of Molecular Weight of DNABiophysical Journal, 1963