Studies of cultured human fibroblasts in diabetes mellitus: changes in heparan sulfate
- 1 January 1979
- journal article
- research article
- Published by American Diabetes Association in Diabetes
- Vol. 28 (1) , 61-64
- https://doi.org/10.2337/diabetes.28.1.61
Abstract
Since the expression of a diabetic gene (or genes) may allow accumulation of substances which might account for some biochemical changes in diabetes mellitus. The incorporation of [35S]sulfate into glycosaminoglycans was studied in cultures of normal and diabetic skin fibroblasts. Heparan sulfate was determined by column chromatography after enzymatic degradation of chondroitin sulfates and dermatan sulfate by chondroitinase ABC. Cultured skin fibroblasts from both insulin-dependent and noninsulin-dependent diabetics had increased proportions of heparan sulfate in the media relative to the other sulfated glycosaminoglycans.This publication has 8 references indexed in Scilit:
- Insulin-dependent childhood diabetes. Normal viability of cultured fibroblastsDiabetes, 1978
- Chronologic and Physiologic Age Affect Replicative Life-Span of Fibroblasts from Diabetic, Prediabetic, and Normal DonorsScience, 1978
- Effect of a platelet endoglycosidase on cell surface associated heparan sulphate of human cultured endothelial and glial cellsThrombosis Research, 1977
- Abnormalities in proliferation and protein synthesis in skin fibroblast cultures from patients with diabetes mellitusDiabetes, 1977
- The anticoagulant effect of heparan sulfate and dermatan sulfateThrombosis Research, 1976
- Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin SulfatesJournal of Biological Chemistry, 1968
- A modified uronic acid carbazole reactionAnalytical Biochemistry, 1962
- STUDIES ON CELL LINES DEVELOPED FROM THE TISSUES OF PATIENTS WITH GALACTOSEMIAThe Journal of Experimental Medicine, 1961