Evaluation of the oxolinic acid - esculin - azide medium for the isolation and enumeration of faecal streptococci in a routine monitoring programme for bathing waters

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Abstract
M-Enterococcus agar (m-Ent) has been generally considered the reference medium for faecal streptococci in bathing waters. However, it shows several shortcomings, and therefore it is important to test newly developed media that can guarantee more precise results. In this sense, the recently described oxolinic acid - esculin - azide agar medium (OAA) and m-enterococccus agar (m-Ent) were comparatively evaluated for the detection of faecal streptococci from seawater and fresh water. The OAA medium showed a significantly higher relative recovery percentage and specificity for both types of water than m-Ent. A similar spectrum of species was recorded from both media, Enterococcus faecium being predominant in fresh water and Enterococcus faecalis, in seawater. The superior performance of the OAA medium in both types of bathing waters, added to the fact that it does not require the use of complementary confirmative tests, makes this medium an excellent candidate to be employed for monitoring programmes.Key words: faecal streptococci, water, monitoring, oxolinic acid - esculin - azide medium, m-enterococcus medium.

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