A HISTOCHEMICAL STUDY OF "ACID" AND "ALKALINE" PHOSPHATASE DISTRIBUTION IN NORMAL HUMAN BONE MARROW SMEARS

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Abstract
1. Three minute fixation in formol vapor at 44 C, followed by 15 minute washing proved to be the most satisfactory fixation procedure for both "acid" and "alkaline" phosphatase technics as applied to bone marrow smears. 2. For both technics a relation between staining intensity and cellular richness was found. 3. The reaction of normal human bone marrow cells to both phosphatase technics is described. Both are predominantly nuclear in location. Nuclear pattern approached that observed with common staining methods and Feulgen’s reaction. Cytoplasmic reaction was nearly negative. Nonspecific and specific granules do not stain after the "alkaline" technic. Nonspecific granules are negative for "acid" phosphatase, while specific neutrophilic are variable, and eosinophilic, constantly positive. Nucleoli are negative after the "acid" technic, being positive for the "alkaline" enzyme. Mitotic chromosomes are positive for both technics. "Acid" phosphatase reaction in cytoplasmic zones of lymphocytes, erythroblasts, plasmacytes and megakaryocytes, is described.

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