Human ??-Enolase: Two-Site Immunoradiometric Assay with a Single Monoclonal Antibody
- 1 January 1987
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 48 (1) , 22-28
- https://doi.org/10.1111/j.1471-4159.1987.tb13122.x
Abstract
A monoclonal antibody (mAb), termed BBS/NC/VI-H14 (H14), that reacts with the human enzyme γγ-enolase was prepared. It was directed against the γ-subunit and did not cross-react with the α- or β-subunit. The mAb H14 can be used for quantitative determination of γγ-enolase in a two-site immunoradiometric assay (two-site IRMA). It is also suitable for immunostaining formalin-fixed tissues. The specific identification of γγ-enolase provided by the two-site IRMA with H14 is discussed in relation to the cellular distribution of this protein.Keywords
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