Sweeping on a microchip: Concentration profiles of the focused zone in micellar electrokinetic chromatography
- 1 September 2001
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 22 (16) , 3509-3513
- https://doi.org/10.1002/1522-2683(200109)22:16<3509::aid-elps3509>3.0.co;2-z
Abstract
On‐line sample concentration by sweeping was investigated in microchip micellar electrokinetic chromatography (MEKC), By changing the distance between the injection cross and the detection points, the profile of the concentration process and the diffusion process in sweeping was elucidated. Rhodamine B injected for 4 s was best concentrated by sweeping at 9.4 mm from the injection cross and the enhancement factor was 450. At the longer distance from this point the peak of Rhodamine B was broadened and diluted by diffusion. The diffusion constant of Rhodamine B calculated from the experiment was 5.7×10–6 cm2s–1. The mixture of rhodamine B, sulforhodamine B, and cresyl fast violet was concentrated by sweeping and separated by MEKC at the same time.Keywords
This publication has 12 references indexed in Scilit:
- Electrokinetic Injection for Stacking Neutral Analytes in Capillary and Microchip ElectrophoresisAnalytical Chemistry, 2001
- Approaching a Million-Fold Sensitivity Increase in Capillary Electrophoresis with Direct Ultraviolet Detection: Cation-Selective Exhaustive Injection and SweepingAnalytical Chemistry, 2000
- Electrokinetic chromatographyJournal of Chromatography A, 1999
- Exceeding 5000-Fold Concentration of Dilute Analytes in Micellar Electrokinetic ChromatographyScience, 1998
- Determination of metal cations in microchip electrophoresis using on-chip complexation and sample stackingJournal of Microcolumn Separations, 1998
- Microchip electrophoresis with sample stackingElectrophoresis, 1995
- Diode array detection in capillary electrophoresisElectrophoresis, 1994
- Optical improvements of a Z-shaped cell for high-sensitivity UV absorbance detection in capillary electrophoresisAnalytical Chemistry, 1993
- Nanoliter-scale multireflection cell for absorption detection in capillary electrophoresisAnalytical Chemistry, 1991
- Rectangular capillaries for capillary zone electrophoresisAnalytical Chemistry, 1990