23 Na NUCLEAR MAGNETIC RESONANCE RELAXATION STUDIES OF SODIUM ION INTERACTION WITH SOLUBLE RNA
- 1 March 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 62 (3) , 644-649
- https://doi.org/10.1073/pnas.62.3.644
Abstract
Interactions between (23)Na(+) and soluble RNA in aqueous solution are studied with the use of (23)Na nuclear magnetic resonance. At low concentrations of NaCl, the interactions obey a simple equilibrium model with a formation constant log (K(f))(3) = 2.8 +/- 0.3. The relaxation rate of the bound sodium is found to be T(1B) (-1) = 222 +/- 19 sec(-1) compared to that of free sodium T(1F) (-1) = 17.5 sec(-1). At high NaCl concentrations, the system deviates from the model, possibly owing to aggregation of the soluble RNA.Keywords
This publication has 7 references indexed in Scilit:
- Proton Magnetic Resonance of Transfer RNAScience, 1968
- 35 C1 NMR studies of zinc adenosine diphosphate complexesBiochemical and Biophysical Research Communications, 1967
- Halide ions as probes for nuclear magnetic resonance studies of proteins. The sulfhydryl groups of hemoglobinJournal of the American Chemical Society, 1967
- NMR Evidence for Complexing of Na+ in Muscle, Kidney, and Brain, and by Actomyosin. The Relation of Cellular Complexing of Na+ to Water Structure and to Transport KineticsThe Journal of general physiology, 1967
- Nuclear Magnetic Resonance Studies of Antibody-Hapten Interactions Using a Chloride Ion Probe*Biochemistry, 1967
- Nuclear spin resonance evidence for complexing of sodium in frog skinBiochimica et Biophysica Acta (BBA) - Biomembranes, 1967
- Halide ions as chemical probes for NMR studies of proteins.Proceedings of the National Academy of Sciences, 1966