PAPER CHROMATOGRAPHIC DETERMINATION OF INDOLES IN HUMAN CEREBROSPINAL FLUID

Publisher Website
Google Scholar
Abstract
Pools of human cerebrospinal fluid of normal protein and cell content were assayed for indoles by extraction with acetone-ether after saturation with sodium chloride, evaporation of the extract to dryness, and paper chromatography of the dissolved residue. The extractions were performed at two different pH values. The indoles were made visible on the chromatogram by spraying with a modified Ehrlich reagent. The following indoles could be detected in human cerebrospinal fluid: try-ptamine, indole-3-acetic acid, tryptophan, and 5-hydroxy-indole-3-acetic acid. The presence of the first two compounds does not appear to have been established previously. No unidentified indole spots were observed.