Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes
Top Cited Papers
- 8 February 2005
- journal article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 11 (3) , 241-247
- https://doi.org/10.1261/rna.7240905
Abstract
MicroRNAs (miRNAs) are short endogenous RNAs known to post-transcriptionally repress gene expression in animals and plants. A microarray profiling survey revealed the expression patterns of 175 human miRNAs across 24 different human organs. Our results show that proximal pairs of miRNAs are generally coexpressed. In addition, an abrupt transition in the correlation between pairs of expressed miRNAs occurs at a distance of 50 kb, implying that miRNAs separated by <50 kb typically derive from a common transcript. Some microRNAs are within the introns of host genes. Intronic miRNAs are usually coordinately expressed with their host gene mRNA, implying that they also generally derive from a common transcript, and that in situ analyses of host gene expression can be used to probe the spatial and temporal localization of intronic miRNAs.Keywords
This publication has 38 references indexed in Scilit:
- Microarray-based, high-throughput gene expression profiling of microRNAsNature Methods, 2004
- MicroRNA maturation: stepwise processing and subcellular localizationThe EMBO Journal, 2002
- Identification of Tissue-Specific MicroRNAs from MousePublished by Elsevier ,2002
- An Abundant Class of Tiny RNAs with Probable Regulatory Roles in Caenorhabditis elegansScience, 2001
- An Extensive Class of Small RNAs in Caenorhabditis elegansScience, 2001
- Identification of Novel Genes Coding for Small Expressed RNAsScience, 2001
- Characterization of the apoptosis-associated tyrosine kinase (AATYK) expressed in the CNSOncogene, 2001
- Characterization of an alternatively spliced AATYK mRNA: Expression pattern of AATYK in the brain and neuronal cellsOncogene, 2001
- Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNANature, 2000
- Expression of the imprinted genesMEST/Mest in human and murine placenta suggests a role in angiogenesisDevelopmental Dynamics, 2000