Nucleic acid (cDNA) and amino acid sequences of alpha-type gliadins from wheat (Triticum aestivum).

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Abstract
The complete amino acid sequence for an .alpha.-type gliadin protein of wheats (T. aestivum Linnaeus) endosperm was derived from a cloned cDNA sequence. An additional c[complementary]DNA clone that corresponds to about 75% of a similar .alpha.-type gliadin was sequenced and shows some important differences. About 97% of the composite sequence of A-gliadin (an .alpha.-type gliadin fraction) was also obtained by direct amino acid sequencing. This sequence shows a high degree of similarity with amino acid sequences derived from both cDNA clones and is virtually identical to one of them. On the basis of sequence information, after loss of the signal sequence, the mature .alpha.-type gliadins may be divided into 5 different domains, 2 of which may have evolved from an ancestral gliadin gene, whereas the remaining 3 contain repeating sequences that may have developed independently.