The Measurement of Ribulose 1, 5-bisphosphate Carboxylase/Oxygenase Concentration in the Leaves of Potato Plants by Enzyme Linked Immunosorbtion Assays
- 1 February 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 39 (2) , 157-164
- https://doi.org/10.1093/jxb/39.2.157
Abstract
Catt, J. W. and Millard, P. 1988. The measurement of ribulose 1, 5-bisyphosphate carboxylase/ oxygenase concentration in the leaves of potato plants by enzyme linked immunosorbtion assays.—J. exp. Bot. 39: 157-164. An enzyme linked immunosorbtion assay (ELISA) has been developed for the measurement of ribulose l, 5-bistfphosphate carboxylase/oxygenase (RUBISCO) concentrations in potato leaves. Extracts of leaves were made using a buffer containing detergent and used to coat ELISA plates. The amount of RUBISCO binding was estimated using a double antibody technique. Various methods of extraction were used and assessed and the efficiency of the assay was tested using purified RUBISCO. The method is applicable to the determination of RUBISCO from diverse sources.Keywords
This publication has 1 reference indexed in Scilit:
- Large-scale isolation of fraction 1 leaf protein (18S) from lucerne (Medicago sativaL)The Journal of Agricultural Science, 1976