Flow cytometric analysis of cellular endogenous fluorescence simultaneously with emission from exogenous fluorochromes, light scatter and absorption
Open Access
- 1 July 1981
- Vol. 2 (1) , 39-43
- https://doi.org/10.1002/cyto.990020109
Abstract
A cytofluorimeter capable of simultaneous 4‐parameter analysis (i.e., endogenous fluorescence (NAD(P)H), exogenous fluorescent dyes, absorption and small angle scatter) is described. Analyses have been performed on isolated rat liver cells, white blood cells, bone marrow, thymus cells and yeast under different metabolic states. This allows correlations between cell function and concentration of cell substances, size, refractive indices, etc. Kinetic measurements are also possible.Keywords
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