Abnormal amino acid metabolism in mutants of Saccharomyces cerevisiae affected in the initiation of sporulation

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Abstract
Amino acid metabolism was examined during sporulation in a wild‐type strain of Saccharomyces cerevisiae and in a mutant homozygous for the spdl mutation (derepression of sporulation). In the wild‐type initiation of sporulation involves a rise in intracellular glutamate. In the derepressed mutant there was no further increase of intracellular glutamate: glutamate was already present at a greater concentration than in the wild‐type. These elevated glutamate levels are apparently due to reduction in the activity of 2‐oxoglutarate dehydrogenase. One consequence of the elevated glutamate levels in diploids homozygous for the spdl mutation is the production of considerable amounts of glycyl‐l‐proline, glutathione and saccharopine.