Gamma-aminobutyric acid uptake by rat kidney brush-border membrane vesicles
- 1 August 1989
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 45 (8) , 726-728
- https://doi.org/10.1007/bf01974569
Abstract
Brush-border membrane vesicles (BBMV) from rat kidney cortex possessed two uptake systems for γ-aminobutyric acid (GABA), a high affinity system (Km=10.9 μM) and a low affinity system (Km=1203 μM). Both uptake systems were inhibited by p-hydroxymercuribenzoic acid and ouabain, and by the action of neuraminidase, whereas the GABA analogs nipecotic acid, β-alanine, 2,4-diaminobutyric acid and 4,5,6,7-tetrahydroisoxazolo-[4,5c]-pyridin-3-ol had no effect on the GABA uptake activity. The BBMW uptake systems were clearly different from the GABA transport systems present in brain tissue.This publication has 9 references indexed in Scilit:
- A Comparative Study and Partial Characterization of Multi-Uptake Systems for ?-Aminobutyric AcidJournal of Neurochemistry, 1987
- Role of sialic acid in synaptosomal transport of amino acid transmitters.Proceedings of the National Academy of Sciences, 1987
- Uptake of γ‐Aminobutyric Acid by Brain Tissue Preparations: A ReevaluationJournal of Neurochemistry, 1986
- A γ-aminobutyric acid-specific transport mechanism in mammalian kidneyBiochimica et Biophysica Acta (BBA) - Biomembranes, 1985
- LIGAND: A versatile computerized approach for characterization of ligand-binding systemsAnalytical Biochemistry, 1980
- Detectability of high and low affinity uptake systems for GABA and glutamate in rat brain slices and synaptosomesLife Sciences, 1973
- Determination of protein: A modification of the lowry method that gives a linear photometric responseAnalytical Biochemistry, 1972
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951