• 1 January 1980
    • journal article
    • research article
    • Vol. 41  (2) , 183-186
Abstract
Cattle were inoculated with a Virginia isolate of A. marginale Theiler and served as an infective source for laboratory-reared Dermacentor andersoni Stiles and D. variabilis (Say) nymphs. Transstadial transmission of A. marginale was demonstrated by feeding the newly molted adult ticks of both species on susceptible cattle and by inoculation of gut and salivary gland homogenates collected from adult ticks at postattachment days 5 and 7. Oral secretions collected from the same group of ticks and inoculated into a susceptible cow did not cause infection. Fluorescent antibody studies of infected tick gut and salivary glands were positive, although smears of tick oral secretions were negative for fluorescence.