Depression of B-lymphocyte levels in the peripheral blood of cows with mastitis

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Abstract
Levels of B[bone marrow-derived]-lymphocytes in peripheral blood of normal and [bacterial] mastitic cows were evaluated by fluorescent-antibody and erythrocyte-antibody-complement[EAC]-rosetting techniques. Normal cows (N [number of cows tested] = 8) had 26.9 .+-. 4.4% surface membrane immunoglobulin-positive lymphocytes, whereas mastitic cows (N = 6) had 16.3 .+-. 3.6% similar cells among lymphocyte preparations purified by the Ficoll-Paque density gradient separation and carbonyl iron phagocytosis methods. Studies on similar lymphocyte preparations by EAC-rosetting techniques showed that normal cows (N = 12) had 33.5 .+-. 7.1% EAC-rosetting lymphocytes whereas mastitic cows (N = 6) had 20.5 .+-. 6.0% similar cells.