Carboxylesterase aus Rinderlebermikrosomen, II. Dissoziation und Assoziation, Molekulargewicht, Reaktion mit E 600
- 1 January 1967
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 348 (Jahresband) , 1115-1119
- https://doi.org/10.1515/bchm2.1967.348.1.1115
Abstract
The carboxylesterase from beef liver was chromatographed on a column of DEAE-Sephadex. Two enzymatlcally active peaks were obtained. The determination of molecular weight (gel filtration on Sephadex G-200) showed that the 2 peaks represented a monomeric and dimeric form of the enzyme. The molecular weight of the dimer, as determined by ultracentrifugation, was 167,000. The stoichiometry of the reaction with E 600 shows that the dimeric form has 2 active centers and the monomer has 1 active center.This publication has 3 references indexed in Scilit:
- Carboxylesterase aus Rinderlebermikrosomen, I. Isolierung, Eigenschaften und SubstratspezifitätHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1967
- Reaction of a microsomal esterase from hog-liver with diethyl phosphateBiochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1966
- DAS MOLEKULARGEWICHT DER MIKROSOMALEN LEBERESTERASE1965