A Method for Improved Resolution for Fluorescence Microscopy Using Plastic-Embedded Material Subjected to Resin Extraction
- 1 January 1990
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 65 (5) , 259-261
- https://doi.org/10.3109/10520299009105616
Abstract
A protocol is given that uses NaOH, benzene, acetone and methanol to extract epoxy resins from semithin sections. Such sections appear superior to paraffin or unsectioned materials for fluorescence microscopic observations. Use of ultrarapid films (e.g., Kodak T-Max P3200) at ISO 3200 minimizes fading without use of antifading agents and without introducing unacceptable photographic grain size.This publication has 3 references indexed in Scilit:
- Scanning electron microscopic observations on deembedded biological tissue sections: Comparison of different fixatives and embedding materialsJournal of Electron Microscopy Technique, 1985
- A critical evaluation of the specificity of aniline blue induced fluorescenceProtoplasma, 1978
- The Use of an Optical Brightener in the Study of Plant StructureStain Technology, 1975