Sulfhydryls—A New Factor in Frost Resistance. III. Relation of SH Increase During Hardening to Protein, Glutathione, & Glutathione Oxidizing Activity

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Abstract
The SH content of Saxifraga chloroplasts shows the same rise during early hardening as does that of the homogenate. Infiltration with GSH fails to increase the SH content of cabbage leaves appreciably, due to oxidation to GSSG. The GSH-oxidising activity increases during hardening, in spite of the rise in total SH. The percentage SH of total possible SH (if all the SS were converted to SH) increases during hardening in spite of homogenate when blended in air to SH content when blended in N2, increases during hardening, again in spite of the increased GSH-oxidizing activity. All these results agree in showing that the SH increase during hardening is due to protein SH and not to glutathione SH.