Effects of Hachimijiogan, Tokishakuyakusan and Keishibukuryogan on Progesterone Secretions by Corpus Luteum

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Abstract
The 27-day-old received 20 IU of PMS subcutaneously, and 56 hours later, 40 IU of hCG to induce superovulated ovaries. Seven days after the hCG treatment, the ovaries were resected and incubated or perifused with an extract Hachimijiogan (T-7), Tokishakuyakusan (TJ-23) or Keishibukuryogan (TJ-25). Progesterone in media was assayed by radioimmunoassay. In the incubation study, TJ-23 showed no remarkable effect. In the perifusion study, TJ-23 or TJ-25 augmented progesterone levels, whereas TJ-7 revealed no effect. These results suggest that TJ-23 or TJ-25 stimulates the corpus luteum to secrete progesterone.

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