Efficacy of Vaccination against Vibriosis in Cultured Kuruma PrawnsPenaeus japonicus

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Abstract
Cultured kuruma prawns were experimentally vaccinated against vibriosis with formalin-killed Vibrio sp. Application of the vaccine by injection, immersion, and spray techniques all reduced mortality of the prawns when they were challenged by Vibrio injection 30 d later. Hemocytes in contact with blood homogenate from immunized prawns produced a chemokinetic factor that activated other hemocytes to migrate through a membrane in a Boyden chamber.