Determination of Soluble Fibrin by Turbidimetry of its Protamine Sulphate-Induced Paracoagulation

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Abstract
Protamine sulphate-induced aggregation of soluble fibrin causes an increase of turbidity in the plasma sample, which can be measured by means of kinetic turbidimetry. A method was developed which is sufficently sensitive for the determination of soluble fibrin in plasma without interfering with the sensitivty for fibrinogen. The performance of the assay was studied by analysing plasma samples with high concentrations of fibrinogen and soluble fibrin at different pH values, at different concentrations of plasma and protamine sulphate, and using different wavelengths and analysis times. Measurements of thrombin-induced fibrinogen-fibrin-transformation by the developed turbidimetric method, gave results that correlated well with the release of fibrinopeptide A. The new protamine sulphate method for turbidimetric determination of soluble fibrin in characterized by its practiability, rapid availability of reproducible, quantitative results, and its economy of reagents and time in single and serial analysis. Therefore it seems well-suited for the routine diagnosis of hypercoagulability with increased fibrinogen turnover.