Use of Serology and Thin-Layer Chromatography for the Assembly of an Authenticated Collection of Serovars Within the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum Complex

Abstract
The seroagglutination test devised by Schaefer and the procedure of Brennan et al. were used to assemble an authenticated collection composed of 30 of the 31 known serovars of of the M.-M. intracellulare-M. scrofulaceum complex and from these serovars a new set of absorbed specific homologous rabbit antisera was prepared. Isolates of a few serovars (serovars 18, 41 and 43) showed the expected seroagglutination reactions but also produced several TLC profiles, a phenomenon which places further emphasis on the need to use TLC and serological analyses when the epidemiological aspects of nontuberculous mycobacteria are examined.