5S RNA‐Protein complex is involved in ribosomal subunit association
- 7 March 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 153 (1) , 125-127
- https://doi.org/10.1016/0014-5793(83)80131-8
Abstract
The immobilized tRNA-50 S ribosomal subunit protein (TP50) complex binds the smaller ribosomal subunit. We constructed tRNA · TP50·5 S [32P] RNA and tRNA·TP50·t [32P] RNA complexes and investigated the accessibility of the 32P-labelled tRNAs to ribonuclease T1. It was found that in this complex both 5 S RNA and tRNA are attacked by T1 RNase. In sharp contrast, the addition of 30 S subunit protects 5 S RNA as well as tRNA from degradation. We suggest that 5 S RNA-TP50 complex is exposed to the ribosomal interface and is involved in subunit interaction.Keywords
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