Simultaneous Determination of Carbohydrates and Products of Carbohydrate Metabolism in Fermentation Mixtures by HPLC

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Abstract
An improved procedure for separating and quantitating carbohydrates, alcohols, and organic acids in fermentation mixtures metabolized by intestinal microflora is described. The high-pressure liquid chromatographic method is efficient, reproducible, and sensitive. A column packed with cationexchange resin in the hydrogen form, eluted isocratically with 0.028 M H2SO4 at 40°C separates the compounds of interest. The eluate is monitored with ultraviolet and refractive index detectors in series. On-line acquistion and storage of detector output by a computer allows post-analysis data manipulation and quantitation. Using this method, the metabolic profiles for the fermentation of glucose, fructose, lactose, and sucrose by several intestinal microorganisms are characterized and compared.