Functional proteomics of circadian expressed proteins from Chlamydomonas reinhardtii
- 22 January 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 559 (1-3) , 129-135
- https://doi.org/10.1016/s0014-5793(04)00051-1
Abstract
In this study, functional proteomics was successfully applied for the characterization of circadian expressed, basic proteins. For this purpose, we have chosen the green model alga Chlamydomonas reinhardtii since its entire nuclear genome is available and it is ideally suited for biochemical enrichment procedures. Proteins from cells harvested during subjective day and night were heparin affinity purified. They were separated by two-dimensional gel electrophoresis suited for basic proteins and analyzed after tryptic digestion by electrospray ionization mass spectrometry. We can show for the first time that the expressions of a protein disulfide isomerase-like protein and a tetratricopeptide repeat protein change in a circadian manner. Interestingly, both proteins are known to be interaction partners in multiprotein complexes including RNA binding proteins.Keywords
This publication has 29 references indexed in Scilit:
- Proteomics of Chlamydomonas reinhardtii Light-Harvesting ProteinsEukaryotic Cell, 2003
- The Clf1p Splicing Factor Promotes Spliceosome Assembly through N-terminal Tetratricopeptide Repeat ContactsPublished by Elsevier ,2003
- Characterization of a Plant Homolog of Hop, a Cochaperone of Hsp90Plant Physiology, 2003
- CHLAMYDOMONAS AS A MODEL ORGANISMAnnual Review of Plant Biology, 2001
- The tetratricopeptide repeat domains of human, tobacco, and nematode PEX5 proteins are functionally interchangeable with the analogous native domain for peroxisomal import of PTS1-terminated proteins in yeastMolecular Genetics and Genomics, 2001
- The Protein Disulfide Isomerase-like RB60 Is Partitioned between Stroma and Thylakoids in Chlamydomonas reinhardtii ChloroplastsJournal of Biological Chemistry, 2001
- Interactions of p60, a mediator of progesterone receptor assembly, with heat shock proteins hsp90 and hsp70Molecular Endocrinology, 1996
- Two‐dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimensin (IPG‐Dalt): The state of the art and the controversy of vertical versus horizontal systemsElectrophoresis, 1995
- Protein disulphide isomerase: building bridges in protein foldingTrends in Biochemical Sciences, 1994
- The WD‐40 repeatFEBS Letters, 1992