Protein kinase CK2 modulates apoptosis induced by resveratrol and epigallocatechin-3-gallate in prostate cancer cells
Open Access
- 1 March 2007
- journal article
- Published by American Association for Cancer Research (AACR) in Molecular Cancer Therapeutics
- Vol. 6 (3) , 1006-1012
- https://doi.org/10.1158/1535-7163.mct-06-0491
Abstract
Resveratrol and epigallocatechin-3-gallate (EGCG) are important candidates as chemopreventive agents by virtue of their ability to induce apoptosis in cancer cells. Casein kinase 2 (CK2) is a ubiquitous protein ser/thr kinase that plays diverse roles in cell proliferation and apoptosis. We have previously shown that overexpression of CK2 suppresses apoptosis induced by a variety of agents, whereas down-regulation of CK2 sensitizes cells to induction of apoptosis. We therefore investigated whether or not CK2 played a role in resveratrol and EGCG signaling in androgen-sensitive (ALVA-41) and androgen-insensitive (PC-3) prostate cancer cells. Resveratrol- and EGCG-induced apoptosis is associated with a significant down-regulation of CK2 activity and protein expression in both the ALVA-41 and PC-3 cells. Overexpression of CK2α protected prostatic cancer cells against resveratrol- and EGCG-induced apoptosis. Relatively low doses (10 μmol/L) of resveratrol and EGCG induced a modest proliferative response in cancer cells that could be switched to cell death by moderate inhibition of CK2. These findings characterize, for the first time, the effects of polyphenolic compounds on CK2 signaling in androgen-sensitive and androgen-insensitive prostatic carcinoma cells and suggest that resveratrol and EGCG may mediate their cellular activity, at least in part, via their targeting of CK2. Further, the data hint at the potential of using these polyphenols alongside CK2 inhibitors in combination chemotherapy. [Mol Cancer Ther 2007;6(3):1006–12]Keywords
This publication has 44 references indexed in Scilit:
- Protein Kinase Casein Kinase 2 Mediates Inhibitor-κB Kinase and Aberrant Nuclear Factor-κB Activation by Serum Factor(s) in Head and Neck Squamous Carcinoma CellsCancer Research, 2006
- Inhibition of survival signalling by dietary polyphenols and indole-3-carbinolEuropean Journal Of Cancer, 2005
- Protein kinase CK2 phosphorylates and upregulates Akt/PKBCell Death & Differentiation, 2005
- Casein Kinase 2 Regulates Both Apoptosis and the Cell Cycle Following DNA Damage Induced by 6-ThioguanineClinical Cancer Research, 2005
- Modulation of phosphatidylinositol‐3‐kinase/protein kinase B‐ and mitogen‐activated protein kinase‐pathways by tea polyphenols in human prostate cancer cellsJournal of Cellular Biochemistry, 2003
- CK2 Is a C-Terminal IκB Kinase Responsible for NF-κB Activation during the UV ResponseMolecular Cell, 2003
- Phosphorylation of the PTEN Tail Acts as an Inhibitory Switch by Preventing Its Recruitment into a Protein ComplexJournal of Biological Chemistry, 2001
- A Potential Role of Nuclear Matrix-associated Protein Kinase CK2 in Protection against Drug-induced Apoptosis in Cancer CellsJournal of Biological Chemistry, 2001
- The Tumor Suppressor PTEN Is Phosphorylated by the Protein Kinase CK2 at Its C TerminusJournal of Biological Chemistry, 2001
- Growth Inhibition, Cell-Cycle Dysregulation, and Induction of Apoptosis by Green Tea Constituent (-)-Epigallocatechin-3-gallate in Androgen-Sensitive and Androgen-Insensitive Human Prostate Carcinoma CellsToxicology and Applied Pharmacology, 2000