Synthesis of cellulose by Acetobacter xylinum. 1. Micromethod for the determination of celluloses

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Abstract
A technique for the purification and quantitative recovery of small samples of bacterial cellulose is descr. A micromethod for cellulose is based on the dissolution of the purified polysaccharide by acetolytic degradation, the conversion of the product into reducing hexose by acid hydrolysis, and the colorimetric estimation of the formed sugar. The applicability of the technique to bacterial celluloses and cotton cellulose was verified. The method is relatively rapid and specific and is appropriate to serial analysis. The lower threshold is 20 [mu]g.; the error range is [plus or minus] 4%.

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