Rapid purification of fructose-1,6-bisphosphate aldolase from spinach chloroplasts
- 1 January 1988
- journal article
- research article
- Published by Springer Nature in Photosynthesis Research
- Vol. 15 (1) , 57-65
- https://doi.org/10.1007/bf00054988
Abstract
A rapid procedure for the purification of fructose-1,6-bisphosphate aldolase from spinach chloroplasts is presented which involves two steps; precipitation of bulk protein with polyethylene glycol and partitioning of remaining soluble protein in aqueous two-phase systems. A 94% pure preparation is obtained within 6h with a yield of 19%. A marked difference in the partition behaviour of the aldolase activity from whole leaf tissue suggested that the procedure is less efficient when leaf extract is used as starting material.Keywords
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