Periodic-Acid-Foot Stain for Connective Tissue

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Abstract
A marked increase in reticular argyrophilia may be obtained in the Foot ammoniated Ag carbonate technique by interposing a strong periodic acid oxidation, 4% aqueous for 2 hrs. at 25.27[degree] C, prior to silvering. Sections so oxidized before the Ag bath show a histological picture of connective tissue that is stronger than that given by the original technique. Stroma of lymphoid tissues (but not other types) is further intensified by brief (5-10 sec.) passage through aqueous 1.5% uranium nitrate after oxidation but before Ag impregnation. The specific action of periodic acid (cleavage of the 1,2-glycol linkage to produce aldehyde radicals) strengthens the premise that the carbonyl radical plays an important part in the phenomenon of connective tissue argyrophilia.