The Nucleoprotein Content of Whole Nuclei

Publisher Website
Google Scholar
Abstract
Measurements by photometric methods of cytological sections of fixed tissues show these to contain 6.5 to 20 times as much protein as nucleic acid. This is a much higher proportion than has been reported in nuclei and chromosomes when isolated for gross chemical analysis. As tissues kept in physiological saline for 3 hrs. before fixation lose about 3/4 of the protein of the nuclei and 1/2 of that of the cytoplasm, it is suggested that the deficiency of protein in gross studies is, at least in certain methods, lost in the isolation of material for gross analysis. In the large amt. of protein of whole nuclei there is probably very little more histone, however, than in the same type of nucleus after isolation.

This publication has 3 references indexed in Scilit: