Soluble factors stimulating secretory protein translocation in bacteria and yeast can substitute for each other.
Open Access
- 1 June 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (11) , 3723-3727
- https://doi.org/10.1073/pnas.84.11.3723
Abstract
MRNA for prepro-.alpha.-factor (pp.alpha.), a yeast secretory glycoprotein, was translated in a wheat germ cell-free system that was posttranslationally supplemented either with inverted vesicles from the plasma membrane of Escherichia coli (INV) or with microsomes from Saccharomyces cerevisiae. A postribosomal supernatant (PRS) from E. coli was found to stimulate translocation of pp.alpha. across the INV membrane. A yeast PRS could substitute for its E. coli counterpart. Likewise, an E. coli PRS could substitute for a yeast PRS and stimulate translocation of pp.alpha. across yeast microsomal membranes.Keywords
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