The Biotransformation of Propylene to Propylene Oxide byMethylococcus Capsulatus(Bath): 1. Optimization of Rates
- 1 January 1992
- journal article
- research article
- Published by Taylor & Francis in Biocatalysis
- Vol. 6 (3) , 163-175
- https://doi.org/10.3109/10242429209014893
Abstract
The rates of propylene oxidation to propylene oxide by Methylococcus capsulatus (Bath) have been optimized in small shake flasks to establish the potential of this process for industrial application. It was shown that addition of the electron donors methanol, formaldehyde, formate or hydrogen stimulated the endogenous rate of propylene oxide formation 10 to 50 fold. Rates in excess of 500 nmol min-1 mg dry weight cells-1 have been obtained using methanol as the donor. These high rates could only be sustained for 3 to 4 min before loss of biocatalytic activity caused the rate of propylene oxide production to decline.Keywords
This publication has 13 references indexed in Scilit:
- Formate transport, growth inhibition and the membrane protonmotive force in two methylotrophs (T15 and L3)Applied Microbiology and Biotechnology, 1987
- Epoxidation of alkenes by alkene-grown Xanthobacter spp.Applied Microbiology and Biotechnology, 1986
- Inactivation of alkene oxidation by epoxides in alkene-and alkane-grown bacteriaApplied Microbiology and Biotechnology, 1985
- Stereospecific formation of 1,2-epoxypropane, 1,2-epoxybutane and 1-chloro-2,3-epoxypropane by alkene-utilizing bacteriaEnzyme and Microbial Technology, 1985
- Production of epoxides from gaseous alkenes by resting-cell suspensions and immobilized cells of alkene-utilizing bacteriaApplied Microbiology and Biotechnology, 1984
- Copper stress underlies the fundamental change in intracellular location of methane mono-oxygenase in methane-oxidizing organisms: Studies in batch and continuous culturesBiotechnology Letters, 1983
- Microbial oxidation of gaseous hydrocarbons: Oxidation of lowerN-alkenes andN-alkanes by resting cell suspensions of various methylotrophic bacteria, and the effect of methane metabolitesFEMS Microbiology Letters, 1980
- Biotransformation of hydrocarbons and related compounds by whole organism suspensions of methane-grown Methylosinus trichosporium OB 3bBiochemical and Biophysical Research Communications, 1979
- The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compoundsBiochemical Journal, 1977
- The acetylene reduction technique as an assay for nitrogenase activity in the methane oxidizing bacterium Methylococcus capsulatus strain bathArchiv für Mikrobiologie, 1976