Ligand Kinetics in Hemoglobin Hiroshima
Open Access
- 1 August 1971
- journal article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 50 (8) , 1772-1775
- https://doi.org/10.1172/jci106666
Abstract
Hemoglobin Hiroshima is an electrophoretically fast-moving variant with a fourfold increase in oxygen affinity and a decreased Bohr effect. Based on a decreased rate of dissociation of O2 in the presence of dithionite and an increased rate of binding of CO by the deoxy form, we have concluded that the kinetic basis of the high affinity exhibited by Hb Hiroshima is the concurrence of a faster combination rate and a slower dissociation rate for ligands.Keywords
This publication has 13 references indexed in Scilit:
- Spin-labelled haemoglobins: A structural interpretation of electron paramagnetic resonance spectra based on X-ray analysisJournal of Molecular Biology, 1971
- The Binding of Hemoglobin to Haptoglobin and Its Relation to Subunit Dissociation of HemoglobinJournal of Biological Chemistry, 1971
- Stereochemistry of Cooperative Effects in Haemoglobin: Haem–Haem Interaction and the Problem of AllosteryNature, 1970
- Kinetics of the reaction of hemoglobin with ethylisocyanide. Interpretation of the results within a dimer scheme.1970
- The Reaction of Oxygen with Hemoglobin and the Kinetic Basis of the Effect of Salt on Binding of OxygenJournal of Biological Chemistry, 1970
- A practical automatic data acquisition system for stopped-flow spectrophotometryComputers and Biomedical Research, 1969
- Hemoglobin Hiroshima (β143 histidine → aspartic acid): a newly identified fast moving beta chain variant associated with increased oxygen affinity and compensatory erythremiaJournal of Clinical Investigation, 1969
- Oxygen-equilibrium characteristics of abnormal hemoglobin Hiroshima (α2β2143 Asp)Archives of Biochemistry and Biophysics, 1968
- Tyrosine Ionization in Human Carbon Monoxide and Deoxyhemoglobins*Biochemistry, 1966
- Apparatus for rapid and sensitive spectrophotometryBiochemical Journal, 1964