Temperature-sensitive mutants of Chandipura virus. I. Inter- and intragroup complementation

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Abstract
Fifty temperature-sensitive (ts) mutants of Chandipura virus, a human rhabdovirus, were classified into 6 complementation groups, designated ChI, ChII, ChIII, ChIV, ChV and ChVI and containing 44, 2, 1, 1, 1 and 1 mutants, respectively. Weak complementation was observed within group ChI, allowing the division of the group into subgroups ChIA and ChIB. Intragroup complementation was most extensive within subgroup ChIB, and 1 mutant in this subgroup complemented all but 1 (ts Ch598) of the mutants in group ChI. If ts Ch598 had been omitted from the analysis the number of complementation groups would have been increased to 7. Consequently, in circumstances where intragenic and intergenic complementation cannot be clearly distinguished, the number of complementation groups identified in rhabdoviruses could be overestimated. The identification of 6 complementation groups in 3 different rhabdoviruses need not imply the existence of an as yet unidentified 6th virus-specified polypeptide. The extensive intragroup complementation observed in Chandipura virus suggests that the functional form of at least one of the virion proteins of Chandipura virus is a multimer.