Effects of Actinomycin D and 2,4-Dinitrophenol on the Development of Root Primordia in Azuki Bean Stem Cuttings

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Abstract
In the rooting of disbudded azuki bean stem cuttings, actinomycin D and 2,4- dinitrophenol (DNP) acted as auxin synergists. Although cuttings treated with actinomycin D or DNP alone formed almost the same number of roots as water-treated cuttings, cuttings treated with actinomycin D or DNP for 24 hr then with auxin for another 24 hr formed more roots than cuttings treated only with auxin during the second 24 hr. Both actinomycin D and DNP increased the number of root primordia with longitudinally divided cells, but they acted differently on the first transverse cell division which led to root primordium formation. Actinomycin D delayed the start of the first transverse cell division, but DNP hastened it.

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