Penetration of 59Fe through Isolated Cuticles of Citrus Leaves1
Open Access
- 1 August 1976
- journal article
- Published by American Society for Horticultural Science in HortScience
- Vol. 11 (4) , 417-419
- https://doi.org/10.21273/hortsci.11.4.417
Abstract
Discs (2cm diameter) from freshly-harvested leaves of ’Pineapple’ orange (Citrus sinensis (L.) Osbeck) were incubated in darkness at 26 ± 2° for 72 hours in a solution containing of 4% pectinase, 0.1% cellulase, 0.1% hemicellulase and 0.2M ethylenediamine tetracetic acid (EDTA) in 2N acetate buffer, pH3.8, to isolate cuticles. Each cuticle was used as a partition between two smaU chambers, with 59Fe in one chamber and distlled water in the other. Samples were taken from the water side to determine 59Fe penetration through isolated cuticle. Greater penetration of 59Fe into cuticles and intact citrus leaves was obtained with 59FeEDTA and through adaxial surfaces than any other 59Fe formulation tested.Keywords
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