Substance P-induced inhibition of Leydig cell steroidogenesis in primary culture
- 1 October 1991
- journal article
- Published by Hindawi Limited in Andrologia
- Vol. 23 (5) , 325-327
- https://doi.org/10.1111/j.1439-0272.1991.tb02572.x
Abstract
Summary. The effect of substance P (SP) on hamster Leydig cell steroidogenesis in primary culture was investigated. Purified Leydig cells were cultured with or without SP for 24 h. The levels of testosterone and progesterone in the culture media were 174 ± 20 pgml−1 and 105 ± 12 pgml−1, respectively. In the presence of SP (10−7 moll−1), testosterone concentration significantly decreased to 123 ± 19 pg ml−1, e.g. with 29.3%. By contrast, at the concentration used, SP had no effect on progesterone secretion. The possible molecular mechanism of the SP action on Leydig cell function was discussed. The reported results indicate that SP can modulate Leydig cell steroidogenesis in culture.Keywords
This publication has 15 references indexed in Scilit:
- Expression of cytochrome P-45017α, 3β-hydroxysteroid dehydrogenase/Δ5 → 4-isomerase, and steroid 5α-reductase in rat H540 Leydig tumor cellsMolecular and Cellular Endocrinology, 1990
- Gonadotropin receptor occupancy and stimulation of cAMP and testosterone production by purified Leydig cells: Critical dependence on cell concentrationMolecular and Cellular Endocrinology, 1990
- On the mechanisms involved in the inhibitory and stimulating actions of transforming growth factor-β on porcine testicular steroidogenesis: an in vitro studyMolecular and Cellular Endocrinology, 1989
- Fibroblast growth factor is a regulator of testosterone secretion in cultured immature Leydig cellsMolecular and Cellular Endocrinology, 1988
- β-Endorphin Production by the Fetal Leydig Cell: Regulation and Implications for Paracrine Control of Sertoli Cell Function*Endocrinology, 1988
- A TGFβ‐Like Peptide Is a Possible Intratesticular Modulator of SteroidogenesisAnnals of the New York Academy of Sciences, 1987
- Transforming growth factor-β inhibits Leydig cell steroidogenesis in primary cultureBiochemical and Biophysical Research Communications, 1987
- Somatomedin C/insulin-like growth factor 1 as a possible intratesticular regulator of Leydig cell activityMolecular and Cellular Endocrinology, 1987
- Hormonal Regulation of the Differentiation of Cultured Ovarian Granulosa Cells*Endocrine Reviews, 1984
- ACTH ZVND B-ENDORPHIN-RELATED PEPTIDES ARE PRESENT IN MULTIPLE SITES IN THE REPRODUCTIVE TRACT OF THE MALE RATEndocrinology, 1982