Selective inhibition of the mitochondrial permeability transition pore at the oxidation‐reduction sensitive dithiol by monobromobimane
Open Access
- 3 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 362 (2) , 239-242
- https://doi.org/10.1016/0014-5793(95)00256-9
Abstract
In this paper we introduce monobromobimane, a thiol reagent, as a selective blocker of the recently identified dithiol whose oxidation‐reduction status modifies voltage sensing by the mitochondrial permeability transition pore, a cyclosporin A‐sensitive channel. Monobromobimane does not inhibit the phosphate carrier, nor does it interfere with Ca2+ transport, energy coupling or ATP production and transport. We show that monobromobimane selectively prevents the shift in pore gating potential caused by some dithiol oxidants or crosslinkers but not by increasing [Ca2+], allowing a clear distinction of the pore agonists which act at this site.Keywords
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