Expression of the isopentenyl transferase gene is regulated by auxin in transgenic tobacco tissues
- 1 January 1996
- journal article
- research article
- Published by Springer Nature in Transgenic Research
- Vol. 5 (1) , 57-65
- https://doi.org/10.1007/bf01979922
Abstract
The isopentenyl transferase gene (ipt) fromAgrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using theAgrobacterium tumefaciens-mediated gene transfer system. The expression of theipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. The addition of auxin to the media rapidly reduced the level of cytokinins in the transgenic tissues and this was associated with a reduction in IPT mRNA and protein levels. It is concluded that the hormone auxin can regulate expression of a gene involved in biosynthesis of the second hormone cytokinin. Although exogenous benzyladenine did not directly affectipt gene expression, it did antagonize the effect of auxin on levels of cytokinins and IPT mRNA and protein.Keywords
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