Malic and Lactic Dehydrogenase Isozymes of Normal and Leukemic Leukocytes Separated on Glass Bead Columns

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Abstract
Five LDH and two MDH isozyme bands were obtained with acrylamide gel electrophoresis of leukocyte extracts. Normal lymphocytes showed a high total H-LDH (heart type) activity (67 per cent) with 25 per cent in LDH-1 and only 3.5 per cent in LDH-5. Lymphocytes from chronic lymphatic leukemia (CLL) and lymphosarcoma leukemia (LSA-LL) had less LDH-1, and more LDH-3 and LDH-4, than normal lymphocytes. The H-LDH fell to 60.5 per cent in CLL and 56 per cent in LSA-LL. PMN leukocytes had low H-LDH activity (38.8 per cent) with 3.3 per cent in LDH-1 and 25.8 per cent in LDH-5. In myelogenous leukemia, myeloblasts had the most LDH-1 and H-LDH, while mature PMN had the least. PMN leukocytes isolated from CLL, LSA-LL, and myelogenous leukemia had LDH patterns like the normal. Monocytes from acute monocytic leukemia were low in LDH-1 and LDH-5, but had a high total enzyme content. They evidently were rich in LDH-2, 3, and 4. Lymphocytes had less MDH-1 (60 per cent) than PMN leukocytes (78 per cent). In CLL, lymphocyte MDH-2 increased. In myelogenous leukemia, myeloblasts had the most MDH-2 and mature PMN the least. Monocytes from monocytic leukemia contained a little more MDH-2 than PMN leukocytes. In general, white cell immaturity and/or ability to divide was associated with high levels of LDH-1, total H-LDH, and MDH-2.

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