Complete amino acid sequence of rat L-type pyruvate kinase deduced from the cDNA sequence
Open Access
- 1 January 1986
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 154 (2) , 465-469
- https://doi.org/10.1111/j.1432-1033.1986.tb09420.x
Abstract
CDNA clones, containing the entire coding region of rat L‐type pyruvate kinase, were isolated and their nucleotide sequences were determined by the dideoxy‐chain‐termination method. The predicted coding region, which spans 543 amino acids, established the complete amino acid sequence of the L‐type isozyme of pyruvate kinase for the first time. The deduced amino acid sequence of the L type has one phosphorylation site in its amino terminus and shows about 68% and 48% homologies with M1‐type pyruvate kinase of chicken and yeast pyruvate kinase respectively. Domain A exhibits higher homology than domains B and C. The residues in the active site of the L‐type enzyme of rats, lying between domains B and A2, are rather different from those of the M1‐type enzyme of chickens, but other residues constituting the active site are identical with those of the chicken M1 type except for one amino acid substitution.This publication has 39 references indexed in Scilit:
- Cyclic AMP as a transcriptional inhibitor of upper eukaryotic gene transcriptionBiochemical and Biophysical Research Communications, 1984
- Muscle and liver pyruvate kinases are closely related: amino acid sequence comparisonsFEBS Letters, 1984
- Triose phosphate isomerase, pyruvate kinase and other α/β-barrel enzymesTrends in Biochemical Sciences, 1983
- Peptide structures of pyruvate kinase isozymesBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982
- Peptide structures of pyruvate kinase isozymesBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982
- One gene, but two messenger RNAs encode liver L and red cell L′ pyruvate kinase subunitsNature, 1981
- Phosphorylation of Human Erythrocyte Pyruvate Kinase by Soluble Cyclic‐AMP‐Dependend Protein KinasesEuropean Journal of Biochemistry, 1980
- Crystal structure of cat muscle pyruvate kinase at a resolution of 2.6 ÅJournal of Molecular Biology, 1979
- The subunit structure of rat liver pyruvate kinaseBiochemical and Biophysical Research Communications, 1978
- Amino acid sequence at the phosphorylated site of rat liver pyruvate kinaseBiochemical and Biophysical Research Communications, 1975