Effect of macrophages on interleukin‐2 (IL‐2)‐ and IL‐4‐induced murine lymphokine‐activated killer activity

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Abstract
The enhancing effect of macrophages on interleukin‐2 (IL‐2)‐ and IL‐4‐induced murine lymphokine‐activated killer (LAK) activity was investigated in this study. Peritoneal macrophages significantly enhanced LAK activity generated from accessory cell‐depleted splenic lymphocytes in both IL‐2 and IL‐4 cultures. This effect was dependent on the number of macrophages and was not replaced by a factor derived from macrophages or lymphocytes. Macrophages enhanced IL‐2‐ and IL‐4‐induced LAK activity against both natural killer (NK)‐sensltive (YAC‐1, P388D1) and NK‐resistant (P815) tumor cells. Negative selection or cells with antibodies and complement showed no differences in surface markers between IL‐2 LAK effectors and IL‐4 LAK effectors generated in the presence of macrophages. These results suggest that the same LAK effector subsets can be enhanced by macrophages In either IL‐2 or IL‐4 cultures.

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