A Simplified Method for Determination of Nifedipine in Human Plasma by High Performance Liquid Chromatography
- 1 December 1989
- journal article
- analytical biochemistry-and-clinical-analysis
- Published by Taylor & Francis in Analytical Letters
- Vol. 22 (15) , 2985-3002
- https://doi.org/10.1080/00032718908052411
Abstract
A simplified high performance liquid chromatographic method was developed to determine the levels of nifedipine in human plasma. Nifedipine and the internal standard, 11-ketoprogesterone, are extracted from alkalinized plasma into organic solvent. The organic solvent is then evaporated to total dryness and the analytes are reconstituted with mobile phase and chromatographed. The limit of quantitation is 10 ng/ml using a 1.0 ml plasma sample.Keywords
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