Interaction of the Escherichia coli dnaA initiation protein with the dnaZ polymerization protein in vivo.

Publisher Website
Google Scholar
Abstract
To define in vivo interactions of E. coli DNA replication components, extragenic suppressors of a dnaZ(TS) mutant were isolated. A temperature-sensitive dnaZ mutant, which is defective in polymerization, was plated at 39.degree. C to select temperature-insensitive revertants. Some of these revertants also were cold sensitive, a phenotypic property that facilitated study of the suppressor. Mapping of the cold sensitivity indicated that some of the suppressor mutations are intragenic but others are located within the initiation gene, dnaA. The dnaA mutations that suppress the dnaZ(TS) defect are designated dnaA(SUZ, CS). The dna(SUZ, CS) strains have a defect in DNA synthesis at low temperature that is typical of an initiation defect. The dnaA product, an initiation factor, apparently interacts in vivo with the dnaZ protein, a polymerization.