Binding of Lipase, Amylase and Protease to Intestinal Epithelium as Affected by Carbohydrates and Lectins in Vitro
Open Access
- 1 September 1979
- journal article
- research article
- Published by Springer Nature in Acta Veterinaria Scandinavica
- Vol. 20 (3) , 329-342
- https://doi.org/10.1186/bf03546595
Abstract
Intraluminal digestive enzymes were shown to bind to chick intestinal epithelial surface (glycocalyx). Affinity of the intestinal epithelium for the enzymes decreased in the order, lipase > amylase > protease. The plant hemagglutinins, Con A, phytohemagglutinin, pokeweed mitogen and raw soybean effectively released lipase and amylase from the glycocalyx. Based on specific inhibition of binding by sugars, such as fucose and N-acetylated sugars, lipase and amylase appeared to be bound to blood group antigen-like sugar moieties on the glycocalyx of the microvilli.Keywords
This publication has 13 references indexed in Scilit:
- Identification of the dietary lectin, wheat germ agglutinin, in human intestinal contentsGastroenterology, 1978
- Enzymic Activities in the Pancreas, Digestive Tract and Feces of Rats Fed Raw or Heated Soy FlourJournal of Nutrition, 1976
- USE OF PLANT AGGLUTININS IN CHARACTERIZATION OF GLYCOPROTEINS AND GLYCOLIPIDS FROM MAMMALIAN CELLS*Annals of the New York Academy of Sciences, 1974
- Cytochemistry of enterocytes and of other cells in the mucous membrane of the small intestine.1974
- The Biochemistry of Plant Lectins (Phytohemagglutinins)Annual Review of Biochemistry, 1973
- FORMATION OF CELL COAT MATERIAL FOR THE WHOLE SURFACE OF COLUMNAR CELLS IN THE RAT SMALL INTESTINE, AS VISUALIZED BY RADIOAUTOGRAPHY WITH L-FUCOSE-3HThe Journal of cell biology, 1970
- Surface Sugar in the IntestineThe Lancet Healthy Longevity, 1969
- HemagglutininsPublished by Elsevier ,1969
- PURIFICATION OF A TOXIC SUBSTANCE FROM DEFATTED SOY BEAN FLOURJournal of Biological Chemistry, 1952
- Identification of Hemagglutinin of Jack Bean with Concanavalin AJournal of Bacteriology, 1936